Hotspot

rbcL-accD

LSC · 57397-58084 · 688 bp

Back to Paeonia

Pi 0.0104

MarkerSeek score 64.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 46
pi: 0.0104
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	CCACCGTTTTTCCATAGAGC	55.0	50.0	57.7	56.8	812-849 bp	1.0	75.4
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	CCACCGTTTTTCCATAGAGCT	55.0	47.6	57.7	58.3	812-849 bp	1.0	74.7
rbcL-accD_p3	GCTGCTGCTTGTGAAGTATG	CCACCGTTTTTCCATAGAGC	50.0	50.0	57.5	56.8	799-836 bp	0.961538	71.8
rbcL-accD_p4	GCTGCTGCTTGTGAAGTATG	CCACCGTTTTTCCATAGAGCT	50.0	47.6	57.5	58.3	799-836 bp	0.961538	71.1
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	ACCACCGTTTTTCCATAGAGC	55.0	47.6	57.7	58.6	813-850 bp	0.961538	71.1