Hotspot

ndhC-trnV-UAC

LSC · 51253-52251 · 999 bp

Back to Paeonia

Pi 0.00965
MarkerSeek score 62.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
999
variable_sites
53
pi
0.00965
unique_haplotype_count
23
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 919-1159 bp 1.0 83.7
ndhC-trnV-UAC_p2 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 918-1158 bp 1.0 80.9
ndhC-trnV-UAC_p3 CTGGTCCTTTGCTAATCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 922-1162 bp 1.0 80.7
ndhC-trnV-UAC_p4 TCTGGTCCTTTGCTAATCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 923-1163 bp 1.0 80.7
ndhC-trnV-UAC_p5 TGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 921-1161 bp 1.0 80.7