Hotspot

trnS-UGA-psbZ

LSC · 36397-36688 · 292 bp

Back to Paeonia

Pi 0.0132

MarkerSeek score 59.30

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 292
variable_sites: 20
pi: 0.0132
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	GAGAGGCAAATACAACGGGT	55.0	50.0	58.1	57.9	407-421 bp	1.0	85.8
trnS-UGA-psbZ_p2	CAAGACCGGAGCTATCAACC	CAACCATCAGGAGAGGCAAA	55.0	50.0	58.1	57.8	417-431 bp	1.0	84.9
trnS-UGA-psbZ_p3	CAAGACCGGAGCTATCAACC	AGGAGAGGCAAATACAACGG	55.0	50.0	58.1	57.6	409-423 bp	1.0	83.4
trnS-UGA-psbZ_p4	GGTTTTCAAGACCGGAGCTA	GAGAGGCAAATACAACGGGT	50.0	50.0	57.5	57.9	413-427 bp	1.0	82.4
trnS-UGA-psbZ_p5	GGTTTTCAAGACCGGAGCTA	CAACCATCAGGAGAGGCAAA	50.0	50.0	57.5	57.8	423-437 bp	1.0	81.5