Hotspot

petN-psbM

LSC · 29209-30046 · 838 bp

Back to Paeonia

Pi 0.00805

MarkerSeek score 67.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 39
pi: 0.00805
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACAATCAGCCAA	50.0	35.0	58.2	54.0	903-959 bp	1.0	54.3
petN-psbM_p2	CTTGGGCTGCGTTAATGGTA	ACGTAAAAACAATCAGCCAA	50.0	35.0	58.3	54.0	891-947 bp	1.0	53.5
petN-psbM_p3	TTGGGCTGCGTTAATGGTAG	ACGTAAAAACAATCAGCCAA	50.0	35.0	58.3	54.0	890-946 bp	1.0	53.5
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACAATCAGCCAAA	50.0	33.3	58.2	54.9	903-959 bp	1.0	53.2
petN-psbM_p5	GGCTGCGTTAATGGTAGTCT	ACGTAAAAACAATCAGCCAA	50.0	35.0	57.7	54.0	887-943 bp	1.0	53.1