Hotspot

atpH-atpI

LSC · 14027-15203 · 1177 bp

Back to Paeonia

Pi 0.00986

MarkerSeek score 61.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1177
variable_sites: 58
pi: 0.00986
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	CCGGTCATGTTCCTTGGATT	45.0	50.0	58.1	57.9	1306-1320 bp	1.0	82.2
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	CCGGTCATGTTCCTTGGATT	50.0	50.0	57.8	57.9	1323-1337 bp	1.0	81.3
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	CCGGTCATGTTCCTTGGATT	50.0	50.0	58.3	57.9	1318-1332 bp	1.0	80.9
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	TACCGGTCATGTTCCTTGGA	45.0	50.0	58.1	58.4	1308-1322 bp	1.0	80.4
atpH-atpI_p5	CACTCCAGGCCCAATAGAAG	CCGGTCATGTTCCTTGGATT	55.0	50.0	57.7	57.9	1354-1368 bp	1.0	80.2