Hotspot

trnR-UCU-atpA

LSC · 10318-10524 · 207 bp

Back to Paeonia

Pi 0.023

MarkerSeek score 64.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 207
variable_sites: 19
pi: 0.023
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATGAAAGGCGTCCATTGTCT	TCAGGAACAGATGGAACGTT	45.0	45.0	57.5	56.8	315-336 bp	1.0	75.2
trnR-UCU-atpA_p2	ATGAAAGGCGTCCATTGTCT	TTCAGGAACAGATGGAACGT	45.0	45.0	57.5	56.8	316-337 bp	1.0	75.1
trnR-UCU-atpA_p3	ATGAAAGGCGTCCATTGTCT	CGACTAAGACATTCACCGAGG	45.0	52.4	57.5	58.2	364-385 bp	1.0	74.9
trnR-UCU-atpA_p4	ATGAAAGGCGTCCATTGTCT	CGGAAGCCCTTTTGAAAGAAG	45.0	47.6	57.5	57.7	340-361 bp	1.0	74.3
trnR-UCU-atpA_p5	ATGAAAGGCGTCCATTGTCT	CGGAAGCCCTTTTGAAAGAA	45.0	45.0	57.5	56.5	340-361 bp	1.0	72.9