Hotspot

rps16-trnQ-UUG

LSC · 6041-7338 · 1298 bp

Back to Paeonia

Pi 0.0182
MarkerSeek score 73.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1298
variable_sites
133
pi
0.0182
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TATGTCCTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1119-1398 bp 1.0 91.1
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1109-1388 bp 1.0 90.1
rps16-trnQ-UUG_p3 TATGTCCTTCAAGTCGCACG TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.0 57.3 1123-1402 bp 1.0 86.4
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1113-1392 bp 1.0 85.4
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1101-1380 bp 1.0 85.0