Hotspot

trnK-UUU-rps16

LSC · 4227-4949 · 723 bp

Back to Paeonia

Pi 0.0123
MarkerSeek score 63.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
723
variable_sites
50
pi
0.0123
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG GGGGTTTTACATAATTTTGCCT 50.0 36.4 58.6 55.2 799-965 bp 0.923077 45.0
trnK-UUU-rps16_p2 ATCAGTCGCGGTCTTTCAAA GGGGTTTTACATAATTTTGCCT 45.0 36.4 57.8 55.2 874-1040 bp 0.923077 44.9
trnK-UUU-rps16_p3 AGTCGCGGTCTTTCAAACTC GGGGTTTTACATAATTTTGCCT 50.0 36.4 58.5 55.2 871-1037 bp 0.923077 44.8
trnK-UUU-rps16_p4 GTCGCGGTCTTTCAAACTCT GGGGTTTTACATAATTTTGCCT 50.0 36.4 58.5 55.2 870-1036 bp 0.923077 44.8
trnK-UUU-rps16_p5 ATCAGTCGCGGTCTTTCAAA GGGGTTTTACATAATTTTGCCTT 45.0 34.8 57.8 55.9 874-1040 bp 0.923077 44.7