Hotspot

trnH-GUG-psbA

LSC · 79-391 · 313 bp

Back to Paeonia

Pi 0.0451

MarkerSeek score 76.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 47
pi: 0.0451
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CATGGTGGATTCACAACCCA	CGAAGCTCCATCTACAAATGG	50.0	47.6	58.1	56.8	381-402 bp	1.0	73.4
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CGAAGCTCCATCTACAAATGG	55.0	47.6	58.1	56.8	354-375 bp	1.0	73.1
trnH-GUG-psbA_p3	GATTCACAACCCACTGCCTT	CGAAGCTCCATCTACAAATGG	50.0	47.6	58.4	56.8	374-395 bp	1.0	71.1
trnH-GUG-psbA_p4	GCCTTGATCCACTTGGCTAC	CGAAGCTCCATCTACAAATGG	55.0	47.6	58.6	56.8	359-380 bp	1.0	69.2
trnH-GUG-psbA_p5	ATTCACAACCCACTGCCTTG	CGAAGCTCCATCTACAAATGG	50.0	47.6	58.7	56.8	373-394 bp	1.0	68.8