Hotspot

ycf1

SSC · 123381-128993 · 5613 bp

Back to Paederia

Pi 0.00154

MarkerSeek score 45.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5613
variable_sites: 13
pi: 0.00154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGATGTAAGACAGGTCATG	ACCAAGAACAACTGTAGGCA	47.6	45.0	57.1	57.0	289 bp	1.0	57.1
ycf1_p2	ACCCCCTATCCTAGAGCATA	ACCAAGAACAACTGTAGGCA	50.0	45.0	56.0	57.0	245 bp	1.0	56.2
ycf1_p3	ATACCCCCTATCCTAGAGCA	ACCAAGAACAACTGTAGGCA	50.0	45.0	56.0	57.0	247 bp	1.0	56.2
ycf1_p4	TACCCCCTATCCTAGAGCAT	ACCAAGAACAACTGTAGGCA	50.0	45.0	56.0	57.0	246 bp	1.0	56.2
ycf1_p5	TGGGATGTAAGACAGGTCATG	GACCAAGAACAACTGTAGGCA	47.6	47.6	57.1	58.2	290 bp	1.0	56.0