Hotspot

rps2

LSC · 15645-16355 · 711 bp

Back to Paederia

Pi 0.000938

MarkerSeek score 46.80

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.000938
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TCTCTTTGACTACTTGAATCGGA	CAGTTCAGGGTTCCTCGTTT	39.1	50.0	57.0	57.7	860-861 bp	1.0	46.2
rps2_p2	TCTCTTTGACTACTTGAATCGGA	TTTCAGTTCAGGGTTCCTCG	39.1	50.0	57.0	57.5	863-864 bp	1.0	43.9
rps2_p3	TCTCTTTGACTACTTGAATCGGA	AGTTCAGGGTTCCTCGTTTC	39.1	50.0	57.0	57.5	859-860 bp	1.0	43.9
rps2_p4	TCTCTTTGACTACTTGAATCGGA	GTTCAGGGTTCCTCGTTTCT	39.1	50.0	57.0	57.5	858-859 bp	1.0	43.9
rps2_p5	TCTCTTTGACTACTTGAATCGGA	TCAGTTCAGGGTTCCTCGTT	39.1	50.0	57.0	58.6	861-862 bp	1.0	43.6