Hotspot

rps16

LSC · 4913-6009 · 1097 bp

Back to Paederia

Pi 0.000608

MarkerSeek score 46.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1097
variable_sites: 1
pi: 0.000608
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCACTACCCCCTCCCTTTTT	TGAGCCAATGACTATTCCTGA	50.0	42.9	57.9	56.4	1195-1196 bp	1.0	58.4
rps16_p2	ATTCGAAAGCGGTAAAGGGG	TGAGCCAATGACTATTCCTGA	50.0	42.9	58.3	56.4	1255-1259 bp	1.0	57.7
rps16_p3	TACAAGTCACTACCCCCTCC	TGAGCCAATGACTATTCCTGA	55.0	42.9	57.8	56.4	1201-1202 bp	1.0	57.6
rps16_p4	TCACTACCCCCTCCCTTTTT	TTGAGCCAATGACTATTCCTGA	50.0	40.9	57.9	57.2	1196-1197 bp	1.0	56.2
rps16_p5	TACAAGTCACTACCCCCTCC	TTGAGCCAATGACTATTCCTGA	55.0	40.9	57.8	57.2	1202-1203 bp	1.0	55.4