Hotspot

rpl16

LSC · 80648-82089 · 1442 bp

Back to Paederia

Pi 0.000925
MarkerSeek score 41.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1442
variable_sites
2
pi
0.000925
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCAATGCAAAGGACGAAGAA CCCATTAGGTCTTTTGCTCA 40.0 45.0 55.6 55.3 1637-1638 bp 1.0 40.1
rpl16_p2 TCTTTCAATGCAAAGGACGA CCCATTAGGTCTTTTGCTCA 40.0 45.0 55.6 55.3 1641-1642 bp 1.0 40.1
rpl16_p3 TTCAATGCAAAGGACGAAGA CCCATTAGGTCTTTTGCTCA 40.0 45.0 55.6 55.3 1638-1639 bp 1.0 40.1
rpl16_p4 CTTTCAATGCAAAGGACGAAGA CCCATTAGGTCTTTTGCTCA 40.9 45.0 57.5 55.3 1640-1641 bp 1.0 40.1
rpl16_p5 TCTTTCAATGCAAAGGACGAAG CCCATTAGGTCTTTTGCTCA 40.9 45.0 57.5 55.3 1641-1642 bp 1.0 40.1