Hotspot

rpl32-trnL-UAG

SSC · 113146-113798 · 653 bp

Back to Paederia

Pi 0.00204
MarkerSeek score 52.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
653
variable_sites
2
pi
0.00204
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGACAGCGGTAAAAGCTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 778-779 bp 1.0 79.1
rpl32-trnL-UAG_p2 TTGGACAGCGGTAAAAGCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 779-780 bp 1.0 79.1
rpl32-trnL-UAG_p3 TGGACAGCGGTAAAAGCTTT AGCAGCGTGTCTACCAATTT 45.0 45.0 58.0 57.8 771-772 bp 1.0 78.6
rpl32-trnL-UAG_p4 TTGGACAGCGGTAAAAGCTT AGCAGCGTGTCTACCAATTT 45.0 45.0 58.0 57.8 772-773 bp 1.0 78.6
rpl32-trnL-UAG_p5 TGGACAGCGGTAAAAGCTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.0 57.8 776-777 bp 1.0 78.5