Hotspot

rpoB-trnC-GCA

LSC · 26966-27997 · 1032 bp

Back to Paederia

Pi 0.000646

MarkerSeek score 38.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1032
variable_sites: 1
pi: 0.000646
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTCCATCCACTAGC	CCGGATTTGAACTGGGGAAA	52.4	50.0	58.1	58.1	1117 bp	1.0	70.3
rpoB-trnC-GCA_p2	CCCTCATTTCCATCCACTAGC	ACCTTTTGTTGATCAGGCGA	52.4	45.0	58.1	57.7	1140 bp	1.0	68.8
rpoB-trnC-GCA_p3	CCCTCATTTCCATCCACTAGC	CACCTTTTGTTGATCAGGCG	52.4	50.0	58.1	57.7	1141 bp	1.0	68.2
rpoB-trnC-GCA_p4	CCCTCATTTCCATCCACTAGC	AAAAGGATTTGCAGTCCCCC	52.4	50.0	58.1	58.4	1099 bp	1.0	68.1
rpoB-trnC-GCA_p5	AGTTCTTCCGTCAAACCTTGA	CCGGATTTGAACTGGGGAAA	42.9	50.0	57.4	58.1	1198 bp	1.0	67.0