Hotspot

trnS-GCU-trnG-UCC

LSC · 8542-9122 · 581 bp

Back to Paederia

Pi 0.00344

MarkerSeek score 61.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 581
variable_sites: 3
pi: 0.00344
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	CAAACCGAAAGCAAAGACCC	50.0	50.0	58.0	57.9	688-694 bp	1.0	79.9
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	CCAAACCGAAAGCAAAGACC	50.0	50.0	58.0	57.9	689-695 bp	1.0	79.9
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	CAAACCGAAAGCAAAGACCC	50.0	50.0	58.0	57.9	687-693 bp	1.0	79.9
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	CCAAACCGAAAGCAAAGACC	50.0	50.0	58.0	57.9	688-694 bp	1.0	79.9
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	CCGAAAGCAAAGACCCCTTA	50.0	50.0	58.0	57.8	684-690 bp	1.0	79.5