Hotspot

matK-trnK-UUU

LSC · 3421-4111 · 691 bp

Back to Paederia

Pi 0.000965

MarkerSeek score 46.90

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 1
pi: 0.000965
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGAAAGACTGGCTGTTGAGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	56.4	58.2	771 bp	1.0	65.9
matK-trnK-UUU_p2	AGAAAGACTGGCTGTTGAGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	56.4	58.2	770 bp	1.0	65.9
matK-trnK-UUU_p3	AGAAAGACTGGCTGTTGAGA	TTGGGTTGCTAACTCAACGG	45.0	50.0	56.4	58.4	786 bp	1.0	64.1
matK-trnK-UUU_p4	AAGACTGGCTGTTGAGATCT	AACGGTAGAGTACTCGGCTT	45.0	50.0	56.2	58.2	768 bp	1.0	64.1
matK-trnK-UUU_p5	AAGACTGGCTGTTGAGATCT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	56.2	58.2	767 bp	1.0	64.1