Hotspot

trnH-GUG-psbA

LSC · 76-344 · 269 bp

Back to Paederia

Pi 0.0198

MarkerSeek score 52.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 269
variable_sites: 8
pi: 0.0198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCTGCTATAGATGCCCCATC	55.0	55.0	58.1	58.0	323-324 bp	1.0	80.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCTGCTATAGATGCCCCATC	50.0	55.0	57.9	58.0	337-338 bp	1.0	80.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCTGCTATAGATGCCCCATC	50.0	55.0	57.9	58.0	336-337 bp	1.0	80.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCTGCTATAGATGCCCCATC	50.0	55.0	57.9	58.0	338-339 bp	1.0	80.5
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CGTGCGAATCTTGGTATGGA	55.0	50.0	58.1	58.1	386-387 bp	1.0	80.1