Hotspot

ycf1

SSC · 128572-134217 · 5646 bp

Back to Pachysandra

Pi 0.0119

MarkerSeek score 44.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5646
variable_sites: 67
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCACAACTTCTTTCGTTCGGA	TCATGAATCGTCCATTCGAA	42.9	40.0	57.8	54.9	186 bp	1.0	48.4
ycf1_p2	TCACAACTTCTTTCGTTCGGA	TTCATGAATCGTCCATTCGA	42.9	40.0	57.8	54.9	187 bp	1.0	48.3
ycf1_p3	CACAACTTCTTTCGTTCGGAC	TCATGAATCGTCCATTCGAA	47.6	40.0	57.8	54.9	185 bp	1.0	47.8
ycf1_p4	CACAACTTCTTTCGTTCGGAC	TTCATGAATCGTCCATTCGA	47.6	40.0	57.8	54.9	186 bp	1.0	47.8
ycf1_p5	TCACAACTTCTTTCGTTCGGA	TTCATGAATCGTCCATTCGAA	42.9	38.1	57.8	55.7	187 bp	1.0	46.9