Hotspot

ndhD-psaC

SSC · 121766-121876 · 111 bp

Back to Pachysandra

Pi 0.0631

MarkerSeek score 76.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 111
variable_sites: 7
pi: 0.0631
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CCGCAGATATTGGCAAGACT	ACCGATTTCTTGAGTGTCCG	50.0	50.0	58.0	57.9	250-251 bp	1.0	81.3
ndhD-psaC_p2	CCGCAGATATTGGCAAGACT	AAGAGATGTGAATCCGCCTG	50.0	50.0	58.0	57.7	274-275 bp	1.0	79.1
ndhD-psaC_p3	AAGAACCCGCAGATATTGGC	ACCGATTTCTTGAGTGTCCG	50.0	50.0	58.3	57.9	256-257 bp	1.0	79.0
ndhD-psaC_p4	CCGCAGATATTGGCAAGACT	GAGATGTGAATCCGCCTGTC	50.0	55.0	58.0	58.4	272-273 bp	1.0	78.3
ndhD-psaC_p5	CCGCAGATATTGGCAAGACT	GCCTGTCCAACCGATTTCTT	50.0	50.0	58.0	58.5	259-260 bp	1.0	78.1