Hotspot

ndhF-rpl32

SSC · 116631-117767 · 1137 bp

Back to Pachysandra

Pi 0.0282

MarkerSeek score 68.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1137
variable_sites: 31
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCTAAGGGTATGATCCATGCA	AAAAAGCTTTTACCGCTGCC	47.6	45.0	57.6	57.9	1239-1275 bp	1.0	71.8
ndhF-rpl32_p2	GCTAAGGGTATGATCCATGCA	GCCCAAGACCCCTTCTTTTT	47.6	50.0	57.6	58.3	1222-1258 bp	1.0	70.8
ndhF-rpl32_p3	AGCTAAGGGTATGATCCATGC	AAAAAGCTTTTACCGCTGCC	47.6	45.0	57.3	57.9	1240-1276 bp	1.0	69.5
ndhF-rpl32_p4	AGCTAAGGGTATGATCCATGC	GCCCAAGACCCCTTCTTTTT	47.6	50.0	57.3	58.3	1223-1259 bp	1.0	68.5
ndhF-rpl32_p5	GCTAAGGGTATGATCCATGCAT	AAAAAGCTTTTACCGCTGCC	45.5	45.0	58.0	57.9	1239-1275 bp	1.0	66.9