Hotspot

trnN-GUU-ndhF

IRb · 112323-114404 · 2082 bp

Back to Pachysandra

Pi 0.00993

MarkerSeek score 48.20

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2082
variable_sites: 14
pi: 0.00993
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	TCAAAGCGAGCCCTTTCATT	TCGTATCCCCATGAATCCGA	45.0	50.0	58.1	58.0	2136-2825 bp	1.0	84.1
trnN-GUU-ndhF_p2	GGGAACGGGTTACCTACTCT	TCGTATCCCCATGAATCCGA	55.0	50.0	58.1	58.0	2162-2851 bp	1.0	83.7
trnN-GUU-ndhF_p3	ACGACCAGTCAGTTAACAGC	TCGTATCCCCATGAATCCGA	50.0	50.0	57.8	58.0	2063-2752 bp	1.0	83.6
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	TCGTATCCCCATGAATCCGA	50.0	50.0	58.2	58.0	2076-2765 bp	1.0	83.6
trnN-GUU-ndhF_p5	TCAAAGCGAGCCCTTTCATT	CGTATCCCCATGAATCCGAC	45.0	55.0	58.1	57.9	2135-2824 bp	1.0	83.5