Hotspot

rpl14-rpl16

LSC · 84815-84941 · 127 bp

Back to Pachysandra

Pi 0.0315

MarkerSeek score 63.90

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 4
pi: 0.0315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ATCGGCGATTACTAGCTCCT	CTATTTCCATAGCGGCGTCA	50.0	50.0	58.1	57.9	280 bp	1.0	79.3
rpl14-rpl16_p2	ATCGGCGATTACTAGCTCCT	CCATAGCGGCGTCAAAAATG	50.0	50.0	58.1	58.2	274 bp	1.0	79.1
rpl14-rpl16_p3	ATCGGCGATTACTAGCTCCT	ATTTCCATAGCGGCGTCAAA	50.0	45.0	58.1	58.3	278 bp	1.0	78.4
rpl14-rpl16_p4	ATCGGCGATTACTAGCTCCT	TCCATAGCGGCGTCAAAAAT	50.0	45.0	58.1	58.3	275 bp	1.0	78.4
rpl14-rpl16_p5	ATCGGCGATTACTAGCTCCT	TTCCATAGCGGCGTCAAAAA	50.0	45.0	58.1	58.5	276 bp	1.0	76.7