Hotspot

psbB-psbT

LSC · 77700-77897 · 198 bp

Back to Pachysandra

Pi 0.0258

MarkerSeek score 65.90

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 198
variable_sites: 5
pi: 0.0258
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GCTGGTATTGACCCGGATTT	GAACTTTAGGCGGTTCTCGA	50.0	50.0	57.9	57.6	372-376 bp	1.0	78.6
psbB-psbT_p2	GCTGGTATTGACCCGGATTT	TAGTCGGAACTTTAGGCGGT	50.0	50.0	57.9	58.5	378-382 bp	1.0	77.9
psbB-psbT_p3	CCGGATTTGGATGCTCAAGT	GAACTTTAGGCGGTTCTCGA	50.0	50.0	58.2	57.6	360-364 bp	1.0	77.2
psbB-psbT_p4	CCGGATTTGGATGCTCAAGT	TAGTCGGAACTTTAGGCGGT	50.0	50.0	58.2	58.5	366-370 bp	1.0	76.4
psbB-psbT_p5	GCTGGTATTGACCCGGATTT	GGAACTTTAGGCGGTTCTCG	50.0	55.0	57.9	58.6	373-377 bp	1.0	76.4