Hotspot

petA-psbJ

LSC · 66337-67301 · 965 bp

Back to Pachysandra

Pi 0.0167

MarkerSeek score 69.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 16
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTGGCATCTGTTATCCTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	1074-1078 bp	1.0	78.5
petA-psbJ_p2	ACGTGTCCAAGGTCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1099-1103 bp	1.0	75.6
petA-psbJ_p3	CATCTGTTATCCTGGCCCAA	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.3	57.6	1070-1074 bp	1.0	73.1
petA-psbJ_p4	TCCATTACGTGTCCAAGGTC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.2	57.6	1105-1109 bp	1.0	72.8
petA-psbJ_p5	CCATTACGTGTCCAAGGTCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.2	57.6	1104-1108 bp	1.0	72.8