Hotspot

trnF-GAA-ndhJ

LSC · 50803-51526 · 724 bp

Back to Pachysandra

Pi 0.023

MarkerSeek score 66.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 16
pi: 0.023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCAGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	866-877 bp	1.0	83.2
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCAGGTAGA	ATCATCCACGCTTGAAACGA	55.0	45.0	58.0	57.9	901-912 bp	1.0	83.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	830-841 bp	1.0	83.0
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCAGGTAGA	TCATCCACGCTTGAAACGAA	55.0	45.0	58.0	58.1	900-911 bp	1.0	82.8
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGA	50.0	45.0	58.1	57.9	865-876 bp	1.0	82.8