Hotspot

psbC-trnS-UGA

LSC · 37732-37949 · 218 bp

Back to Pachysandra

Pi 0.0413

MarkerSeek score 71.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 218
variable_sites: 9
pi: 0.0413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CATTTGTGGCATGCAGGAAG	GATGGCTCCGGTCTTGAAAA	50.0	50.0	58.0	58.2	393-396 bp	1.0	80.4
psbC-trnS-UGA_p2	CATTTGTGGCATGCAGGAAG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.0	58.2	343-346 bp	1.0	80.3
psbC-trnS-UGA_p3	ATTTGTGGCATGCAGGAAGA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	58.1	58.2	392-395 bp	1.0	79.8
psbC-trnS-UGA_p4	ATTTGTGGCATGCAGGAAGA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.1	58.2	342-345 bp	1.0	79.8
psbC-trnS-UGA_p5	CCTGTTCTTTCCATGACCCC	GATGGCTCCGGTCTTGAAAA	55.0	50.0	58.2	58.2	321-324 bp	1.0	79.5