Hotspot

psbM-trnD-GUC

LSC · 31019-32179 · 1161 bp

Back to Pachysandra

Pi 0.0172
MarkerSeek score 66.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1161
variable_sites
20
pi
0.0172
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAAGAGTGCAGTAGC AGTTCAATCGGTCAGAGCAC 45.0 50.0 55.2 57.9 1274-1296 bp 1.0 58.1
psbM-trnD-GUC_p2 AGAATGAAGAGTGCAGTAGC TACACCTTATTTCCCCGGGA 45.0 50.0 55.2 57.7 1298-1320 bp 1.0 56.6
psbM-trnD-GUC_p3 ACTAGAATGAAGAGTGCAGTAGC AGTTCAATCGGTCAGAGCAC 43.5 50.0 58.0 57.9 1277-1299 bp 1.0 56.6
psbM-trnD-GUC_p4 AGAATGAAGAGTGCAGTAGC GTACACCTTATTTCCCCGGG 45.0 55.0 55.2 57.7 1299-1321 bp 1.0 56.0
psbM-trnD-GUC_p5 AGAATGAAGAGTGCAGTAGC TCCCCGGGATTGTAGTTCAA 45.0 50.0 55.2 58.3 1287-1309 bp 1.0 56.0