Hotspot

petN-psbM

LSC · 29765-30913 · 1149 bp

Back to Pachysandra

Pi 0.0122

MarkerSeek score 63.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1149
variable_sites: 14
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.2	57.0	1308-1311 bp	1.0	64.1
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.6	57.0	1247-1250 bp	1.0	62.5
petN-psbM_p3	CCCTCGTAGTATGGGGAAGA	TGCTACTGCACTCTTCATTCT	55.0	42.9	57.6	57.0	1257-1260 bp	1.0	62.3
petN-psbM_p4	TCCCTCGTAGTATGGGGAAG	TGCTACTGCACTCTTCATTCT	55.0	42.9	57.6	57.0	1258-1261 bp	1.0	62.3
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTCTTCATTC	50.0	45.0	58.2	55.5	1308-1311 bp	1.0	59.7