Hotspot

ycf2

LSC · 87859-94170 · 6312 bp

Back to Pachypleurum

Pi 0.0103
MarkerSeek score 63.40
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
LSC
length_bp
6312
variable_sites
93
pi
0.0103
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 TGCATGTATTAGATATATCCTGTCGA GGCCCGGGAAACAAAGATAA 34.6 50.0 57.5 58.2 260 bp 1.0 42.5
ycf2_p2 TGCATGTATTAGATATATCCTGTCG GGCCCGGGAAACAAAGATAA 36.0 50.0 56.2 58.2 260 bp 1.0 42.5
ycf2_p3 GCATGTATTAGATATATCCTGTCGA GGCCCGGGAAACAAAGATAA 36.0 50.0 56.0 58.2 259 bp 1.0 42.5
ycf2_p4 TGCATGTATTAGATATATCCTGTCGAA GGCCCGGGAAACAAAGATAA 33.3 50.0 58.0 58.2 260 bp 1.0 42.5
ycf2_p5 TGCATGTATTAGATATATCCTGTCGA TGTAACTCACTAGAGGCCCG 34.6 55.0 57.5 58.5 274 bp 1.0 42.3