Hotspot

ycf1

IRb · 109820-111740 · 1921 bp

Back to Pachypleurum

Pi 0.00352

MarkerSeek score 46.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1921
variable_sites: 10
pi: 0.00352
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	AAAATCTGCAGGTAGTGGGC	55.0	50.0	58.0	58.2	2331 bp	0.333333	52.0
ycf1_p2	CCGGAACTTCTTCGTAGTGG	AAAATCTGCAGGTAGTGGGC	55.0	50.0	58.0	58.2	2330 bp	0.333333	52.0
ycf1_p3	CAATTGTGGACTCTCGGGAG	AAAATCTGCAGGTAGTGGGC	55.0	50.0	58.0	58.2	2785 bp	0.333333	51.9
ycf1_p4	ATCTCCCCAGGTAGGATTCG	AAAATCTGCAGGTAGTGGGC	55.0	50.0	58.0	58.2	2487 bp	0.333333	51.9
ycf1_p5	TCAAAGCGAGCCCTTTTCTT	AAAATCTGCAGGTAGTGGGC	45.0	50.0	58.0	58.2	2540 bp	0.333333	51.9