Hotspot

ndhF-rpl32

SSC · 114006-115079 · 1074 bp

Back to Pachypleurum

Pi 0.0195

MarkerSeek score 69.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1074
variable_sites: 28
pi: 0.0195
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	50.0	50.0	58.0	58.2	1207-1259 bp	1.0	84.2
ndhF-rpl32_p2	TGCCGACGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1205-1257 bp	1.0	84.1
ndhF-rpl32_p3	TTGCCGACGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1206-1258 bp	1.0	84.1
ndhF-rpl32_p4	GTTGCCGACGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.0	58.3	1218-1270 bp	1.0	83.0
ndhF-rpl32_p5	AAAAGAAGAAGTCCCGCTCC	TAAGGCTGCCCAATATCCCT	50.0	50.0	57.8	58.2	1195-1247 bp	1.0	83.0