Hotspot

rps11-rpl36

LSC · 80872-80988 · 117 bp

Back to Pachypleurum

Pi 0.0171

MarkerSeek score 63.10

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 117
variable_sites: 3
pi: 0.0171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CCCCTTTGGTATTCTCCGTG	ATTTGTTCCAACCCAAGACA	55.0	40.0	58.0	55.3	240 bp	1.0	59.5
rps11-rpl36_p2	CCCCTTTGGTATTCTCCGTG	TTTGTTCCAACCCAAGACAT	55.0	40.0	58.0	55.3	239 bp	1.0	59.5
rps11-rpl36_p3	CCCCTTTGGTATTCTCCGTG	AATTTGTTCCAACCCAAGACA	55.0	38.1	58.0	56.2	241 bp	1.0	58.0
rps11-rpl36_p4	CCCCTTTGGTATTCTCCGTG	ATTTGTTCCAACCCAAGACAT	55.0	38.1	58.0	55.9	240 bp	1.0	56.2
rps11-rpl36_p5	CCCCTTTGGTATTCTCCGTG	GTTCCAACCCAAGACATAAACA	55.0	40.9	58.0	56.8	236 bp	1.0	55.2