Hotspot

trnL-UAA-trnF-GAA

LSC · 49957-50318 · 362 bp

Back to Pachypleurum

Pi 0.0129

MarkerSeek score 58.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 362
variable_sites: 7
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	ACATGTCAATACCGGCAACA	TCTACCAGCTGAGCTATCCC	45.0	55.0	57.8	58.0	475 bp	1.0	79.6
trnL-UAA-trnF-GAA_p2	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	45.0	50.0	57.8	58.1	511 bp	1.0	79.1
trnL-UAA-trnF-GAA_p3	ACATGTCAATACCGGCAACA	GTGCCAGGAACCAGATTTGA	45.0	50.0	57.8	58.1	526 bp	1.0	78.8
trnL-UAA-trnF-GAA_p4	ACATGTCAATACCGGCAACA	TCAGTCCTCTGCTCTACCAG	45.0	55.0	57.8	57.9	487 bp	1.0	78.5
trnL-UAA-trnF-GAA_p5	ACATGTCAATACCGGCAACA	CATGTGCCAGGAACCAGATT	45.0	50.0	57.8	58.2	529 bp	1.0	78.3