primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	992	1176	252-274	1.000000	84.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	992	1176	266-288	1.000000	84.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	992	1176	265-287	1.000000	84.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	992	1176	267-289	1.000000	84.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TCCTCTAGACCTAGCTGCTG	20	20	55.0	55.0	58.1	57.6	0.0	13.4	0.485584	992	1176	253-275	1.000000	82.5
trnK-UUU_p1	trnK-UUU	1	ACTCCCAAGCACACGAATTT	TGTCCGAGCGGTTTGATTTT	20	20	45.0	45.0	58.0	58.4	0.0	9.0	0.418720	2440	5075	2936-2993	1.000000	79.5
trnK-UUU_p2	trnK-UUU	2	ACTCCCAAGCACACGAATTT	TTGTCCGAGCGGTTTGATTT	20	20	45.0	45.0	58.0	58.4	0.0	9.0	0.418720	2440	5075	2937-2994	1.000000	79.5
trnK-UUU_p3	trnK-UUU	3	ACTCCCAAGCACACGAATTT	TTTGTCCGAGCGGTTTGATT	20	20	45.0	45.0	58.0	58.4	0.0	9.0	0.418720	2440	5075	2938-2995	1.000000	79.5
trnK-UUU_p4	trnK-UUU	4	ACTCCCAAGCACACGAATTT	TTTTGTCCGAGCGGTTTGAT	20	20	45.0	45.0	58.0	58.4	0.0	9.0	0.418720	2440	5075	2939-2996	1.000000	79.5
trnK-UUU_p5	trnK-UUU	5	ACTCCCAAGCACACGAATTT	ATTTTTGTCCGAGCGGTTTG	20	20	45.0	45.0	58.0	57.6	0.0	9.0	0.434441	2440	5075	2941-2998	1.000000	79.4
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCCCTCGCTTCATCCAAATG	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.0	56.5	0.0	13.9	1.591763	5076	5784	814-833	1.000000	70.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCGATGGTATGGACGAATCC	AGGAACTGTTCGGGATCTTT	20	20	55.0	45.0	57.9	56.5	0.0	13.9	1.629363	5076	5784	831-850	1.000000	70.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CGATGGTATGGACGAATCCC	AGGAACTGTTCGGGATCTTT	20	20	55.0	45.0	57.9	56.5	0.0	13.9	1.629363	5076	5784	830-849	1.000000	70.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.2	56.5	20.3	13.9	1.717497	5076	5784	769-788	1.000000	70.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAAGCCGAGTACTCTACCGT	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.2	56.5	20.3	13.9	1.717497	5076	5784	770-789	1.000000	70.0
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10717	10852	209-216	1.000000	69.4
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10717	10852	212-219	1.000000	69.1
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10717	10852	215-222	1.000000	68.6
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10717	10852	218-225	1.000000	68.3
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	10717	10852	213-220	1.000000	66.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	29159	29876	774-777	1.000000	80.3
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	29159	29876	835-838	1.000000	78.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	29159	29876	792-795	1.000000	78.1
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.4	57.6	14.7	0.0	0.706353	29159	29876	853-856	1.000000	76.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	29159	29876	773-776	1.000000	74.4
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATCC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.250489	29967	31102	1310-1344	1.000000	79.0
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATCC	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.450004	29967	31102	1249-1283	1.000000	77.4
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	GCTACTGCACTGTTCATCCT	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.544620	29967	31102	1309-1343	1.000000	76.6
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	GCTACTGCACTGTTCATCCT	20	20	50.0	50.0	57.6	57.6	0.0	0.0	0.744134	29967	31102	1248-1282	1.000000	75.1
petN-psbM_p5	petN-psbM	5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATCC	20	20	50.0	50.0	57.1	57.9	0.0	0.0	0.991926	29967	31102	1311-1345	1.000000	73.0
trnG-UCC-trnfM-CAU_p1	trnG-UCC-trnfM-CAU	1	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.9	58.0	0.0	4.2	0.137348	38246	38439	292-301	1.000000	83.2
trnG-UCC-trnfM-CAU_p2	trnG-UCC-trnfM-CAU	2	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.8	58.0	0.0	4.2	0.208039	38246	38439	263-272	1.000000	83.1
trnG-UCC-trnfM-CAU_p3	trnG-UCC-trnfM-CAU	3	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.124730	38246	38439	328-337	1.000000	82.9
trnG-UCC-trnfM-CAU_p4	trnG-UCC-trnfM-CAU	4	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.195421	38246	38439	299-308	1.000000	82.7
trnG-UCC-trnfM-CAU_p5	trnG-UCC-trnfM-CAU	5	ATGCGGATATGGTCGAATGG	TGAGGTCACGGGTTCAAATC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.278053	38246	38439	290-299	1.000000	82.1
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	ACATGTCAATACCGGCAACA	TCTACCAGCTGAGCTATCCC	20	20	45.0	55.0	57.8	58.0	0.0	17.4	0.195169	49957	50318	475-475	1.000000	79.6
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	20	20	45.0	50.0	57.8	58.1	0.0	20.1	0.244559	49957	50318	511-511	1.000000	79.1
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	ACATGTCAATACCGGCAACA	GTGCCAGGAACCAGATTTGA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.282964	49957	50318	526-526	1.000000	78.8
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	ACATGTCAATACCGGCAACA	TCAGTCCTCTGCTCTACCAG	20	20	45.0	55.0	57.8	57.9	0.0	0.0	0.328962	49957	50318	487-487	1.000000	78.5
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	ACATGTCAATACCGGCAACA	CATGTGCCAGGAACCAGATT	20	20	45.0	50.0	57.8	58.2	0.0	0.0	0.349295	49957	50318	529-529	1.000000	78.3
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	64488	65464	1033-1167	1.000000	75.9
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	64488	65464	1020-1154	1.000000	75.5
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	64488	65464	1034-1168	1.000000	75.2
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	64488	65464	1021-1155	1.000000	74.8
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	64488	65464	1038-1172	1.000000	74.3
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.9	57.9	0.0	8.4	0.175650	67816	67972	239-244	1.000000	85.6
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.252065	67816	67972	231-236	1.000000	85.2
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.9	58.2	0.0	8.4	0.232908	67816	67972	238-243	1.000000	85.1
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	20	20	50.0	45.0	57.9	57.9	6.2	8.4	0.202860	67816	67972	266-271	1.000000	84.6
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	20	20	50.0	45.0	57.9	58.2	6.2	8.4	0.260118	67816	67972	265-270	1.000000	84.2
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	GGTGCCACGGAAAGATATGT	20	20	45.0	50.0	57.9	58.0	5.7	0.0	0.152297	68047	68425	449-449	1.000000	79.7
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CAAAACAAACGCGCTACCAA	GGTGCCACGGAAAGATATGT	20	20	45.0	50.0	58.2	58.0	5.7	0.0	0.209555	68047	68425	450-450	1.000000	79.2
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	TTGTACCCAAAACAAACGCG	GGTGCCACGGAAAGATATGT	20	20	45.0	50.0	57.8	58.0	3.5	0.0	0.228712	68047	68425	457-457	1.000000	79.0
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	AAAACAAACGCGCTACCAAG	GACCTGCTAAAGACCCGAAC	20	20	45.0	55.0	57.9	58.3	5.7	0.0	0.390691	68047	68425	487-487	1.000000	77.7
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TACCCAAAACAAACGCGCTA	GGTGCCACGGAAAGATATGT	20	20	45.0	50.0	58.4	58.0	5.7	0.0	0.447454	68047	68425	454-454	1.000000	77.3
psbT-psbN_p1	psbT-psbN	1	GAGAACCGCCTAAAGTTCCA	TCGAGGAACATGGGGACTAG	20	20	50.0	55.0	57.5	58.2	0.0	0.0	0.690904	75730	75815	138-138	1.000000	76.8
psbT-psbN_p2	psbT-psbN	2	GAGAACCGCCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.708847	75730	75815	139-139	1.000000	76.6
psbT-psbN_p3	psbT-psbN	3	GAGAACCGCCTAAAGTTCCA	GATCCCTTCGAGGAACATGG	20	20	50.0	55.0	57.5	57.8	0.0	0.0	0.714285	75730	75815	145-145	1.000000	76.5
psbT-psbN_p4	psbT-psbN	4	GAGAACCGCCTAAAGTTCCA	GGGTACGCCTTATATACCGC	20	20	50.0	55.0	57.5	57.7	0.0	0.0	0.751829	75730	75815	193-193	1.000000	75.6
psbT-psbN_p5	psbT-psbN	5	GAGAACCGCCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	20	20	50.0	50.0	57.5	57.6	0.0	6.3	0.846011	75730	75815	201-201	1.000000	74.8
rps11-rpl36_p1	rps11-rpl36	1	CCCCTTTGGTATTCTCCGTG	ATTTGTTCCAACCCAAGACA	20	20	55.0	40.0	58.0	55.3	0.0	0.0	2.693490	80872	80988	240-240	1.000000	59.5
rps11-rpl36_p2	rps11-rpl36	2	CCCCTTTGGTATTCTCCGTG	TTTGTTCCAACCCAAGACAT	20	20	55.0	40.0	58.0	55.3	0.0	0.0	2.693490	80872	80988	239-239	1.000000	59.5
rps11-rpl36_p3	rps11-rpl36	3	CCCCTTTGGTATTCTCCGTG	AATTTGTTCCAACCCAAGACA	20	21	55.0	38.1	58.0	56.2	0.0	0.0	2.880162	80872	80988	241-241	1.000000	58.0
rps11-rpl36_p4	rps11-rpl36	4	CCCCTTTGGTATTCTCCGTG	ATTTGTTCCAACCCAAGACAT	20	21	55.0	38.1	58.0	55.9	0.0	0.0	3.105385	80872	80988	240-240	1.000000	56.2
rps11-rpl36_p5	rps11-rpl36	5	CCCCTTTGGTATTCTCCGTG	GTTCCAACCCAAGACATAAACA	20	22	55.0	40.9	58.0	56.8	0.0	0.0	3.232399	80872	80988	236-236	1.000000	55.2
ycf2_p1	ycf2	1	TGCATGTATTAGATATATCCTGTCGA	GGCCCGGGAAACAAAGATAA	26	20	34.6	50.0	57.5	58.2	9.2	29.3	6.677747	91136	91285	260-260	1.000000	42.5
ycf2_p2	ycf2	2	TGCATGTATTAGATATATCCTGTCG	GGCCCGGGAAACAAAGATAA	25	20	36.0	50.0	56.2	58.2	9.2	29.3	6.930097	91136	91285	260-260	1.000000	42.5
ycf2_p3	ycf2	3	GCATGTATTAGATATATCCTGTCGA	GGCCCGGGAAACAAAGATAA	25	20	36.0	50.0	56.0	58.2	9.2	29.3	7.165243	91136	91285	259-259	1.000000	42.5
ycf2_p4	ycf2	4	TGCATGTATTAGATATATCCTGTCGAA	GGCCCGGGAAACAAAGATAA	27	20	33.3	50.0	58.0	58.2	9.2	29.3	7.191080	91136	91285	260-260	1.000000	42.5
ycf2_p5	ycf2	5	TGCATGTATTAGATATATCCTGTCGA	TGTAACTCACTAGAGGCCCG	26	20	34.6	55.0	57.5	58.5	9.2	0.0	7.048680	91136	91285	274-274	1.000000	42.3
ycf1_p1	ycf1	1	CCCGGAACTTCTTCGTAGTG	AAAATCTGCAGGTAGTGGGC	20	20	55.0	50.0	58.0	58.2	0.7	15.1	0.168407	109820	111740	2331-2331	0.333333	52.0
ycf1_p2	ycf1	2	CCGGAACTTCTTCGTAGTGG	AAAATCTGCAGGTAGTGGGC	20	20	55.0	50.0	58.0	58.2	0.0	15.1	0.168407	109820	111740	2330-2330	0.333333	52.0
ycf1_p3	ycf1	3	CAATTGTGGACTCTCGGGAG	AAAATCTGCAGGTAGTGGGC	20	20	55.0	50.0	58.0	58.2	11.8	15.1	0.176312	109820	111740	2785-2785	0.333333	51.9
ycf1_p4	ycf1	4	ATCTCCCCAGGTAGGATTCG	AAAATCTGCAGGTAGTGGGC	20	20	55.0	50.0	58.0	58.2	0.0	15.1	0.177481	109820	111740	2487-2487	0.333333	51.9
ycf1_p5	ycf1	5	TCAAAGCGAGCCCTTTTCTT	AAAATCTGCAGGTAGTGGGC	20	20	45.0	50.0	58.0	58.2	0.0	15.1	0.179320	109820	111740	2540-2540	0.333333	51.9
ycf1-ndhF_p1	ycf1-ndhF	1	CCCTGCCCACACTAGAAAAA	GTGGGCGCATCTCTTCTTAT	20	20	50.0	50.0	57.7	57.8	0.0	10.8	0.533083	111741	111782	854-885	1.000000	79.8
ycf1-ndhF_p2	ycf1-ndhF	2	CCCTGCCCACACTAGAAAAA	GGGGAATAAGAGGATTGGCC	20	20	50.0	55.0	57.7	57.7	0.0	0.0	0.580814	111741	111782	974-1005	1.000000	79.3
ycf1-ndhF_p3	ycf1-ndhF	3	CCCTGCCCACACTAGAAAAA	TGTTCGTTGGATCCCTAGGA	20	20	50.0	50.0	57.7	57.8	0.0	11.5	0.527645	111741	111782	1328-1359	1.000000	78.8
ycf1-ndhF_p4	ycf1-ndhF	4	CCCTGCCCACACTAGAAAAA	AAAATCTGCAGGTAGTGGGC	20	20	50.0	50.0	57.7	58.2	0.0	15.1	0.446941	111741	111782	868-868	0.333333	49.8
ycf1-ndhF_p5	ycf1-ndhF	5	CTCATGAAGGCCTACCGTTG	AAAATCTGCAGGTAGTGGGC	20	20	55.0	50.0	58.3	58.2	5.2	15.1	0.503924	111741	111782	166-166	0.333333	49.3
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGACGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	20	20	50.0	50.0	58.0	58.2	20.5	16.6	0.199227	114006	115079	1207-1259	1.000000	84.2
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGACGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	20	20	45.0	50.0	58.0	58.2	20.5	16.6	0.210516	114006	115079	1205-1257	1.000000	84.1
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGACGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	20	20	45.0	50.0	58.0	58.2	20.5	16.6	0.210516	114006	115079	1206-1258	1.000000	84.1
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGACGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	20	20	50.0	50.0	58.0	58.3	20.5	0.0	0.345950	114006	115079	1218-1270	1.000000	83.0
ndhF-rpl32_p5	ndhF-rpl32	5	AAAAGAAGAAGTCCCGCTCC	TAAGGCTGCCCAATATCCCT	20	20	50.0	50.0	57.8	58.2	0.0	16.6	0.353345	114006	115079	1195-1247	1.000000	83.0