Hotspot

ndhF

SSC · 106782-108998 · 2217 bp

Back to Pachyphytum

Pi 0.00586

MarkerSeek score 53.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 13
pi: 0.00586
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGATGGGAATCAAGAAACTTCG	AGGATCTGGTGAATTAGAAACCA	45.5	39.1	57.4	57.2	2296-2656 bp	1.0	40.5
ndhF_p2	TCTGGATGGGAATCAAGAAACT	AGGATCTGGTGAATTAGAAACCA	40.9	39.1	57.1	57.2	2299-2659 bp	1.0	40.5
ndhF_p3	GGATGGGAATCAAGAAACTTCG	GGATCTGGTGAATTAGAAACCA	45.5	40.9	57.4	55.8	2295-2655 bp	1.0	40.5
ndhF_p4	GGATGGGAATCAAGAAACTTCG	AGGATCTGGTGAATTAGAAACCAT	45.5	37.5	57.4	57.7	2296-2656 bp	1.0	40.5
ndhF_p5	TCTGGATGGGAATCAAGAAACTT	AGGATCTGGTGAATTAGAAACCA	39.1	39.1	57.7	57.2	2299-2659 bp	1.0	40.5