Hotspot

trnC-GCA-petN

LSC · 27319-28185 · 867 bp

Back to Pachyphytum

Pi 0.00839

MarkerSeek score 66.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 7
pi: 0.00839
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	899-932 bp	1.0	81.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAACCAGCCCAAGCAAGA	50.0	45.0	58.1	57.6	905-938 bp	1.0	79.8
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TAAACCAGCCCAAGCAAGAC	50.0	50.0	58.1	58.4	904-937 bp	1.0	79.7
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	917-950 bp	1.0	79.1
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TTAGAGTCCACTTCGTCCCC	50.0	55.0	58.1	58.4	961-994 bp	1.0	79.0