Hotspot

trnG-GCC-trnR-UCU

LSC · 9965-10198 · 234 bp

Back to Pachyphytum

Pi 0.0478

MarkerSeek score 76.50

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 10
pi: 0.0478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	TCCTGAGTTGACGTCGACTA	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	57.8	55.6	324-348 bp	1.0	50.0
trnG-GCC-trnR-UCU_p2	TAGCCTTCCAAGCTACCGAT	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.2	55.6	297-321 bp	1.0	49.9
trnG-GCC-trnR-UCU_p3	TCCTGAGTTGACGTCGACTA	AGAAGACCTATGTCCTATCCA	50.0	42.9	57.8	54.6	318-342 bp	1.0	49.8
trnG-GCC-trnR-UCU_p4	TAGCCTTCCAAGCTACCGAT	AGAAGACCTATGTCCTATCCA	50.0	42.9	58.2	54.6	291-315 bp	1.0	49.7
trnG-GCC-trnR-UCU_p5	TCCTGAGTTGACGTCGACTA	ACCAAAGGTTTAGAAGACCTATGT	50.0	37.5	57.8	57.6	329-353 bp	1.0	49.4