Hotspot

trnS-GCU-trnG-GCC

LSC · 8562-9196 · 635 bp

Back to Pachyphytum

Pi 0.0142
MarkerSeek score 61.70
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
635
variable_sites
9
pi
0.0142
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA TGTTCGGAATACAAAGAAAACCG 50.0 39.1 58.0 57.9 759-761 bp 1.0 56.3
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC TGTTCGGAATACAAAGAAAACCG 50.0 39.1 58.0 57.9 758-760 bp 1.0 56.3
trnS-GCU-trnG-GCC_p3 GCTCGTACAACGGATTAGCA TGTTCGGAATACAAAGAAAACCG 50.0 39.1 58.1 57.9 777-779 bp 1.0 55.7
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA CGAAGGACCCCTTAACTATTAGG 50.0 47.8 58.0 57.8 738-740 bp 1.0 55.5
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC CGAAGGACCCCTTAACTATTAGG 50.0 47.8 58.0 57.8 737-739 bp 1.0 55.5