Hotspot

rpl33-rps18

LSC · 66921-67161 · 241 bp

Back to Pachycladon

Pi 0.0111

MarkerSeek score 60.60

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 5
pi: 0.0111
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACACCCCTAGTCGATTGGAA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	389-407 bp	1.0	82.2
rpl33-rps18_p2	AACACCCCTAGTCGATTGGA	TCAATTCGATCCCCCGATTG	50.0	50.0	58.1	57.7	390-408 bp	1.0	82.1
rpl33-rps18_p3	AAGAATCGGCATAACACCCC	TCAATTCGATCCCCCGATTG	50.0	50.0	57.9	57.7	402-420 bp	1.0	82.0
rpl33-rps18_p4	ATCGGCATAACACCCCTAGT	TCAATTCGATCCCCCGATTG	50.0	50.0	58.2	57.7	398-416 bp	1.0	80.9
rpl33-rps18_p5	AAGAATCGGCATAACACCCC	ATTCGATCCCCCGATTGGAT	50.0	50.0	57.9	58.6	399-417 bp	1.0	79.0