Hotspot

atpB-rbcL

LSC · 53511-54311 · 801 bp

Back to Pachycladon

Pi 0.0145

MarkerSeek score 61.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 21
pi: 0.0145
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	947-951 bp	1.0	73.9
atpB-rbcL_p2	CGAAACCTCTGGATCCGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	889-893 bp	1.0	73.8
atpB-rbcL_p3	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	946-950 bp	1.0	73.6
atpB-rbcL_p4	CGAAACCTCTGGATCCGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	888-892 bp	1.0	73.5
atpB-rbcL_p5	TCCAGTACCGGACCAATGAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	945-949 bp	1.0	73.2