Hotspot

psbZ-trnG

LSC · 35407-35887 · 481 bp

Back to Pachycladon

Pi 0.0188

MarkerSeek score 66.80

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 481
variable_sites: 18
pi: 0.0188
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	TCCTGATGGTTGGTCGAGTA	CGGATAGCGGGAATCGAAC	50.0	57.9	57.8	58.2	625-659 bp	1.0	74.9
psbZ-trnG_p2	TCCTGATGGTTGGTCGAGTA	GATAGCGGGAATCGAACCC	50.0	57.9	57.8	57.8	623-657 bp	1.0	74.7
psbZ-trnG_p3	TCCTGATGGTTGGTCGAGTA	GGATAGCGGGAATCGAACC	50.0	57.9	57.8	57.8	624-658 bp	1.0	74.7
psbZ-trnG_p4	CCGTTGTATTTGCGTCTCCT	CGGATAGCGGGAATCGAAC	50.0	57.9	58.3	58.2	641-675 bp	1.0	74.4
psbZ-trnG_p5	CCGTTGTATTTGCGTCTCCT	GATAGCGGGAATCGAACCC	50.0	57.9	58.3	57.8	639-673 bp	1.0	74.3