Hotspot

psbC-trnS

LSC · 34605-34779 · 175 bp

Back to Pachycladon

Pi 0.0143

MarkerSeek score 61.60

Primer available yes

Label psbC-trnS

Feature Metadata

label_name: psbC-trnS
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 5
pi: 0.0143
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS_p1	GCAGCGGGATTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	259-272 bp	1.0	79.8
psbC-trnS_p2	GCAGCGGGATTTGAAAAAGG	GATGGCTCCGGTCTTGAAAA	50.0	50.0	58.3	58.2	308-321 bp	1.0	79.6
psbC-trnS_p3	TCGTGGGTCATTTATGGCAC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	300-313 bp	1.0	79.6
psbC-trnS_p4	GGCAGCGGGATTTGAAAAAG	GATGGCTCCGGTCTTGAAAA	50.0	50.0	58.3	58.2	309-322 bp	1.0	79.5
psbC-trnS_p5	TCGTGGGTCATTTATGGCAC	GATGGCTCCGGTCTTGAAAA	50.0	50.0	58.3	58.2	349-362 bp	1.0	79.4