Hotspot

trnT-psbD

LSC · 31036-32173 · 1138 bp

Back to Pachycladon

Pi 0.00926

MarkerSeek score 58.70

Primer available yes

Label trnT-psbD

Feature Metadata

label_name: trnT-psbD
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 19
pi: 0.00926
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1281-1303 bp	1.0	82.3
trnT-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1284-1306 bp	1.0	82.1
trnT-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.3	58.0	1258-1280 bp	1.0	80.6
trnT-psbD_p4	GGCCCTTTTAACTCAGTGGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	57.7	58.0	1296-1318 bp	1.0	80.5
trnT-psbD_p5	TGGTAGAGTAACGCCATGGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.4	58.0	1280-1302 bp	1.0	79.3