Hotspot

atpH-atpI

LSC · 13373-13876 · 504 bp

Back to Pachycladon

Pi 0.0175

MarkerSeek score 69.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 504
variable_sites: 16
pi: 0.0175
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	668-676 bp	1.0	81.2
atpH-atpI_p2	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	650-658 bp	1.0	81.1
atpH-atpI_p3	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	55.0	50.0	58.2	58.0	672-680 bp	1.0	80.5
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTTGCCACTTTAGCTGCG	50.0	45.0	57.9	57.9	645-653 bp	1.0	80.4
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	TTTTTGCCACTTTAGCTGCG	50.0	45.0	57.9	57.9	627-635 bp	1.0	80.3