Hotspot

trnG-trnR

LSC · 9278-9430 · 153 bp

Back to Pachycladon

Pi 0.0227

MarkerSeek score 63.80

Primer available yes

Label trnG-trnR

Feature Metadata

label_name: trnG-trnR
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 7
pi: 0.0227
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnR_p1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	275-295 bp	1.0	50.8
trnG-trnR_p2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	281-301 bp	1.0	50.7
trnG-trnR_p3	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	50.0	40.0	57.9	53.9	295-315 bp	1.0	50.3
trnG-trnR_p4	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	50.0	40.0	58.1	53.9	283-303 bp	1.0	50.3
trnG-trnR_p5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	50.0	38.1	58.0	54.8	276-296 bp	1.0	49.8