Hotspot

ycf1

SSC · 126800-132484 · 5685 bp

Back to Pachira

Pi 0.461

MarkerSeek score 74.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5685
variable_sites: 2268
pi: 0.461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAATCAAGCTCTGCTTCCGT	AGCCCTTTTGATTGGATGGG	45.0	50.0	57.8	58.1	443 bp	0.5	57.4
ycf1_p2	AAATCAAGCTCTGCTTCCGT	GCCCTTTTGATTGGATGGGA	45.0	50.0	57.8	58.1	442 bp	0.5	57.4
ycf1_p3	TTTTTACCGACAGGCGAACT	AGCCCTTTTGATTGGATGGG	45.0	50.0	57.8	58.1	688 bp	0.5	57.0
ycf1_p4	CCGTGTTGCTCTTGTATTGC	AGCCCTTTTGATTGGATGGG	50.0	50.0	57.8	58.1	427 bp	0.5	57.0
ycf1_p5	TTTTTACCGACAGGCGAACT	GCCCTTTTGATTGGATGGGA	45.0	50.0	57.8	58.1	687 bp	0.5	57.0