Hotspot

psaC

SSC · 120082-120327 · 246 bp

Back to Pachira

Pi 0.518

MarkerSeek score 76.70

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 118
pi: 0.518
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	CTTCCGCCAGCTGGATTAAT	TGGTTAGGGCCCTTATGTGT	50.0	50.0	58.0	58.3	1049 bp	0.5	57.2
psaC_p2	CTTCCGCCAGCTGGATTAAT	AGGGCCCTTATGTGTCTTGA	50.0	50.0	58.0	58.3	1044 bp	0.5	57.1
psaC_p3	CTTCCGCCAGCTGGATTAAT	GGTTAGGGCCCTTATGTGTC	50.0	55.0	58.0	57.7	1048 bp	0.5	57.0
psaC_p4	GCCGAGTACAGACGTTTCTT	TGGTTAGGGCCCTTATGTGT	50.0	50.0	57.9	58.3	1341 bp	0.5	56.9
psaC_p5	GCCGAGTACAGACGTTTCTT	AGGGCCCTTATGTGTCTTGA	50.0	50.0	57.9	58.3	1336 bp	0.5	56.9