Hotspot

ndhH

SSC · 124871-126052 · 1182 bp

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Pi 0.491

MarkerSeek score 80.40

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 485
pi: 0.491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CAACGAATCGCACGTAGAGA	GAAAACGTCAACGACTGCTG	50.0	50.0	58.1	58.0	1949 bp	0.5	59.1
ndhH_p2	TCAACGAATCGCACGTAGAG	GAAAACGTCAACGACTGCTG	50.0	50.0	58.1	58.0	1950 bp	0.5	59.1
ndhH_p3	CAACGAATCGCACGTAGAGA	GAAGAAAACAAGGGGTCCGT	50.0	50.0	58.1	58.0	2076 bp	0.5	58.9
ndhH_p4	TCAACGAATCGCACGTAGAG	GAAGAAAACAAGGGGTCCGT	50.0	50.0	58.1	58.0	2077 bp	0.5	58.9
ndhH_p5	CAACGAATCGCACGTAGAGA	CGTCAACGACTGCTGTCTTA	50.0	50.0	58.1	58.0	1944 bp	0.5	58.8