Hotspot

ndhG

SSC · 121109-121639 · 531 bp

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Pi 0.509

MarkerSeek score 81.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 259
pi: 0.509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACACATAAGGGCCCTAACCA	AGAATGAGGCCTTTCTCCCT	50.0	50.0	58.3	58.0	1191 bp	0.5	57.2
ndhG_p2	TCAAGACACATAAGGGCCCT	AGAATGAGGCCTTTCTCCCT	50.0	50.0	58.3	58.0	1196 bp	0.5	57.2
ndhG_p3	GACACATAAGGGCCCTAACC	AGAATGAGGCCTTTCTCCCT	55.0	50.0	57.7	58.0	1192 bp	0.5	57.1
ndhG_p4	ACACATAAGGGCCCTAACCA	AGGCCTTTCTCCCTTTCTCT	50.0	50.0	58.3	57.9	1185 bp	0.5	57.0
ndhG_p5	TCAAGACACATAAGGGCCCT	AGGCCTTTCTCCCTTTCTCT	50.0	50.0	58.3	57.9	1190 bp	0.5	56.9